Multi-spectral imaging, which simultaneously captures the spatial and spectral information of a scene, is widely used across diverse fields, including remote sensing, biomedical imaging, and agricultural monitoring. Here, we introduce a snapshot multi-spectral imaging approach employing a standard monochrome image sensor with no additional spectral filters or customized components. Our system leverages the inherent chromatic aberration of wavelength-dependent defocusing as a natural source of physical encoding of multi-spectral information; this encoded image information is rapidly decoded via a deep learning-based multi-spectral Fourier Imager Network (mFIN). We experimentally tested our method with six illumination bands and demonstrated an overall accuracy of 92.98% for predicting the illumination channels at the input and achieved a robust multi-spectral image reconstruction on various test objects. This deep learning-powered framework achieves high-quality multi-spectral image reconstruction using snapshot image acquisition with a monochrome image sensor and could be useful for applications in biomedicine, industrial quality control, and agriculture, among others.

In this paper, we propose an online multi-spectral neuron tracing method with uniquely designed modules, where no offline training are required. Our method is trained online to update our enhanced discriminative correlation filter to conglutinate the tracing process. This distinctive offline-training-free schema differentiates us from other training-dependent tracing approaches like deep learning methods since no annotation is needed for our method. Besides, compared to other tracing methods requiring complicated set-up such as for clustering and graph multi-cut, our approach is much easier to be applied to new images. In fact, it only needs a starting bounding box of the tracing neuron, significantly reducing users' configuration effort. Our extensive experiments show that our training-free and easy-configured methodology allows fast and accurate neuron reconstructions in multi-spectral images.